Random amplified polymorphic DNA analysis of China isolates of Phytophthora parasitica var. nicotianae from different districts
نویسندگان
چکیده
China isolates of Phytophthora parasitica var. Nicotianae have been divided into three large pathogenic groups (PGS) (Hv(A), Iv(B), Wv(C)) which came from Yunnan, Shandong and Liaoning. These three districts belong to main tobacco regions in China: 15 isolates derived from Yunnan of the southwest, 13 isolates from Shandong of the east and 20 isolates from Liaoning of the northeast. PGS were determined through pathogenic assays in greenhouse studies with three tobacco cultivars. The isolates were analyzed by means of the random amplified polymorphic DNA (RAPD) method. All tested isolates could be differentiated into four cluster groups. Each cluster group contained different PGS did not belong to the same cluster group. The conclusion shown that there was no significant different in RAPD pattern among highly virulence, intermediate virulence and weakly virulence groups in China. Therefore, the similarities and difference in handing patterns by RAPD could not be a useful molecular tool in identification and phytogenetic studies of the PGS of the same pathogen from the difference tobacco districts.
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